. 2015 Mar 19;290(20):12614–12629. doi: 10.1074/jbc.M115.642777

TABLE 1.

Mutagenesis primers for introduction of mutations in LDI and LD

Fw, forward primer; Rv, reverse primer.

Primer name	Primer sequence (5′ to 3′)
LDI-ΔV⁵-Fw	`GAGAAAAGAGAGGCTGAAGCTAAGGACGAGTGCCAACCAGGGGT`
LDI-ΔV⁵-Rv	`ACCCCTGGTTGGCACTCGTCCTTAGCTTCAGCCTCTCTTTTCTC`
LDI-R34A-Fw	`CGGGTCTGCGGCGCCGGTCCCAGCCGGCCC`
LDI-R34A-Rv	`GGGCCGGCTGGGACCGGCGCCGCAGACCCG`
LDI-R38A-Fw	`CGCGGTCCCAGCGCGCCCATGCTGGTGAAGG`
LDI-R38A-Rv	`CCTTCACCAGCATGGGCGCGCTGGGACCGCG`
LDI-R38W-Fw	`CGCGGTCCCAGCTGGCCCATGCTGGTGAAGGAG`
LDI-R38W-Rv	`CTCCTTCACCAGCATGGGCCAGCTGGGACCGCG`
LDI-L41G-Fw	`AGCCGGCCCATGGGGGTGAAGGAGCGGTGCTGC`
LDI-L41G-Rv	`GCAGCACCGCTCCTTCACCCCCATGGGCCGGCT`
LDI-L41W-Fw	`AGCCGGCCCATGTGGGTGAAGGAGCGGTGCTGC`
LDI-L41W-Rv	`GCAGCACCGCTCCTTCACCCACATGGGCCGGCT`
LDI-V42D-Fw	`CGGCCCATGCTGGATAAGGAGCGGTGCTGCCGG`
LDI-V42D-Rv	`CCGGCAGCACCGCTCCTTATCCAGCATGGGCCG`
LDI-L41G-V42D-Fw	`AGCCGGCCCATGGGGGATAAGGAGCGGTGCTGCCGG`
LDI-L41G-V42D-Rv	`CCGGCAGCACCGCTCCTTATCCCCCATGGGCCGGCT`
LD-D730W-Fw	`CCAAGTGAAAAGAACGAATGGAATTGGCCCCTGATGAAACC`
LD-D730W-Rv	`GGTTTCATCAGGGGCCAATTCCATTCGTTCTTTTCACTTGG`
LD-D730R-Fw	`CCAAGTGAAAAGAACGAACGTAATTGGCCCCTGATGAAACC`
LD-D730R-Rv	`GGTTTCATCAGGGGCCAATTACGTTCGTTCTTTTCACTTGG`