FIGURE 6.

INSeq analysis of the importance of genes spanning BT3362–BT3380 to the fitness of B. thetaiotaomicron VPI-5482 under different environmental conditions. The frequency (relative abundances) of mutants in the input INSeq library (left column) is compared with their frequency in output communities recovered (i) after 15 or 45 h of growth in TYG medium in a continuously fed batch fermenter; (ii) 10 days after monocolonization of C57BL/6J wild-type, Rag1−/−, or Myd88−/− mice; or (iii) 10 days after colonization of mice with a six-member community of human gut Bacteroidetes, an eight-member community of human gut Firmicutes and Actinobacteria, or a 14-member community composed of the Bacteroidetes, Firmicutes, and Actinobacteria. Note that data for BT3381–BT3384, which are not part of the locus required for 260.8 epitope production, are included as reference controls.