FIGURE 1.

DDI2/3 expression in response to DNA-damaging agents. A, Northern hybridization showing that after MMS treatment, the DDI2/3 transcript level is drastically increased in DBY747 cells. Yeast cells were treated with 0.1% MMS for 48 min prior to RNA isolation and Northern hybridization. Each lane contains 15 μg of total RNA. The blot was stripped and re-hybridized with an ACT1 probe. B–H, induction of DDI2-lacZ by different DNA-damaging agents. The β-gal assays were processed as described under “Experimental Procedures,” and the results after various DNA-damaging agents are shown as fold induction relative to untreated. All data represent the average of at least three experiments with standard deviations.