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DDI2/3 is induced by cyanamide but is not involved in its own transcriptional regulation. A, fold induction of DDI2/3-lacZ by cyanamide. Experimental conditions are as described in Fig. 1, B–H. B, deletion of both DDI2 and DDI3 genes does not affect DDI2/3-lacZ induction by cyanamide or MMS. Black bars, no treatment; open bars, treated with 0.03% MMS; gray bars, treated with 10 mm cyanamide. Experimental conditions are as described in Fig. 1, B–H.
