Fusion activity of SNAREs with alternative proteinaceous membrane anchors. Vacuoles were prepared from cells carrying Vti1, Vam3, or Nyv1 anchored by the TMD of ALP or a synthetic poly-LV sequence. Lipid (A, C, and E) and content (B, D, and F) mixing were assayed in parallel in the presence or absence of ATP. Gdi1 had been added to reactions where indicated to inhibit docking and fusion. Means ± S.D. are shown for three independent experiments.