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. 2015 Mar 27;290(20):12821–12832. doi: 10.1074/jbc.M115.647776

FIGURE 6.

FIGURE 6.

Fusion activity of vacuoles carrying two TMD substitutions. Vacuoles were isolated from cells carrying combinations of Vam3 and Vti1 with the indicated substitutions of their TMDs. A, aliquots were TCA-precipitated and analyzed by SDS-PAGE and Western blotting against Vam3 and Vti1. Mixing of lipid (B) and content (C) was assayed for these vacuoles in parallel in the presence or absence of ATP and Gdi1. Means ± S.D. are shown for three independent experiments.