TABLE 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Refs. |
|---|---|---|
| BJ3505 | MATa pep4::HIS3 prb1-Δ1.6R lys2-208 trp1-Δ101 ura3-52 gal2 can | 67 |
| DKY6281 | MATa pho8::TRP1 leu2-3 leu2-112 lys2-801 suc2-Δ9 trp1-Δ901 ura3-52 | 3 |
| BJ3505 vam3Δ | BJ3505; vam3::TRP | 35 |
| DKY6281 vam3Δ | DKY6281; vam3::HIS | 35 |
| BJ Vam3-WT-HA | BJ3505; vam3::TRP; pRS416-Vam3-WT-HA3 | This study |
| DKY Vam3-WT-HA | DKY6281; vam3::HIS; pRS416-Vam3-WT-HA3 | This study |
| BJ Vam3-LA | BJ3505; vam3:TRP; pCM189-Vam3-LA | This study |
| DKY Vam3-LA | DKY6281; vam3::HIS; pCM189-Vam3-LA | This study |
| BJ Vam3-ALP-HA | BJ3505; vam3::TRP; pRS416-Vam3-ALP-HA3 | This study |
| DKY Vam3-ALP-HA | DKY6281; vam3::HIS; pRS416-Vam3-ALP-HA3 | This study |
| BJ Vam3-LV-HA | BJ3505; vam3::TRP; pRS416-Vam3-LV-HA3 | This study |
| DKY Vam3-LV-HA | DKY6281; vam3::HIS; pRS416-Vam3-LV-HA3 | This study |
| BJ Vam3-LV-GP-HA | BJ3505; vam3::TRP; pRS416-Vam3-LV-GP- HA3 | This study |
| DKY Vam3-LV-GP-HA | DKY6281; vam3::HIS; pRS416-Vam3-LV-GP- HA3 | This study |
| BJ Vam3-JM-HA | BJ3505; vam3::TRP; pRS416-Vam3-JM-HA3 | This study |
| DKY Vam3-JM-HA | DKY6281; vam3::HIS; pRS416-Vam3-JM-HA3 | This study |
| BJ Vam3-PP-HA | BJ3505; vam3::TRP; pRS416-Vam3-PP-HA3 | This study |
| DKY Vam3-PP-HA | DKY6281; vam3::HIS; pRS416-Vam3-PP-HA3 | This study |
| BJ Vam3-plus3-HA | BJ3505; vam3::TRP; p4RS16-Vam3-plus3-HA3 | This study |
| DKY Vam3-plus3-HA | DKY6281; vam3::HIS; pRS416-Vam3-plus3-HA3 | This study |
| BJ Vam3-plus6-HA | BJ3505; vam3::TRP; pRS416-Vam3-plus6-HA3 | This study |
| DKY Vam3-plus6-HA | DKY6281; vam3::HIS; pRS416-Vam3-plus6-HA3 | This study |
| BJ Vti1-WT-HA | BJ3505; vti1::kanMX; pRS416-Vti1-WT-HA3 | This study |
| DKY Vti1-WT-HA | DKY6281; vti1::kanMX; pRS416-Vti1-WT-HA3 | This study |
| BJ Vti1-LA | BJ3505; vti1::kanMX; pRS414-Vti1-LA | This study |
| DKY Vti1-LA | DKY6281; vti1::kanMX; pRS413-Vti1-LA | This study |
| BJ Vti1-WT-HA | BJ3505; vti1::kanMX; pRS414-Vti1-WT- HA3 | This study |
| DKY Vti1-WT-HA | DKY6281; vti1::kanMX; pRS413-Vti1-WT-HA3 | This study |
| BJ Vti1-ALP-HA | BJ3505; vti1::kanMX; pRS414-Vti1-ALP- HA3 | This study |
| DKY Vti1-ALP-HA | DKY6281; vti1::kanMX; pRS413-Vti1-ALP-HA3 | This study |
| BJ Vti1-LV-HA | BJ3505; vti1::kanMX; pRS414-Vti1-LV- HA3 | This study |
| DKY Vti1-LV-HA | DKY6281; vti1::kanMX; pRS413-Vti1-LV- HA3 | This study |
| BJ3505 nvy1Δ | BJ3505; nvy1::TRP1 | 35 |
| DKY6281 nvy1Δ | DKY6281; nyv1::HIS3 | 35 |
| BJ Nyv1-WT | BJ3505; nvy1::TRP1; pRS406-Nyv1-WT | This study |
| DKY Nyv1-WT | DKY6281; nyv1::HIS3; pRS406-Nyv1-WT | This study |
| BJ Nyv1-LA | BJ3505; nvy1::TRP1; pCM189-Nyv1-LA | This study |
| DKY Nyv1-LA | DKY6281; nyv1::HIS3; pCM189-Nyv1-LA | This study |
| BJ Nyv1-ALP | BJ3505; nvy1::TRP1; pRS416-Nyv1-ALP | This study |
| DKY Nyv1-ALP | DKY6281; nyv1::HIS3; pRS416-Nyv1-ALP | This study |
| BJ Nyv1-LV | BJ3505; nvy1::TRP1; pRS406-Nyv1-LV | This study |
| DKY Nyv1-LV | DKY6281; nyv1::HIS3; pRS406-Nyv1-LV | This study |
| BJ Nyv1-LV-GP | BJ3505; nvy1::TRP1; pRS416-Nyv1-LV-GP | This study |
| DKY Nyv1-LV-GP | DKY6281; nyv1::HIS3; pRS416-Nyv1-LV-GP | This study |
| BJ3505 Vti1-WT-HA/Vam3-WT-HA | BJ3505; vti1::kanMX; vam3::NAT, pRS414-endo-Vti1-WT- HA3; pRS416-Vam3-WT-HA3 | This study |
| DKY6281 Vti1-WT-HA/Vam3-WT-HA | DKY6281; vti1::kanMX; vam3::NAT; pRS413-endo-Vti1-WT-HA3; pRS416-Vam3-WT-HA3 | This study |
| BJ3505 Vti1-ALP-HA/Vam3-ALP-HA | BJ3505; vti1::kanMX; vam3::NAT; pRS414-endo-Vti1-ALP-HA3; pRS416-Vam3-ALP-HA3 | This study |
| DKY6281 Vti1-ALP-HA/Vam3-ALP-HA | DKY6281; vti1::kanMX; vam3::NAT; pRS413-endo-Vti1-ALP-HA3; pRS416-Vam3-ALP-HA3 | This study |
| BJ3505 Vti1-ALP-HA/Vam3-LV-HA | BJ3505; vti1::kanMX; vam3::NAT; pRS414-endo-Vti1-ALP-HA3; pRS416-Vam3-LV-HA3 | This study |
| DKY6281 Vti1-ALP-HA/Vam3-LV-HA | DKY6281; vti1::kanMX; vam3::NAT; pRS413-endo-Vti1-ALP-HA3; pRS416-Vam3-LV-HA3 | This study |