TABLE 3.
ITC analysis of interface mutations on ARF7 PB1 domain interaction
Titrations were performed at 25 °C. ND = heat signature was not detected.
| Proteins |
Kd | ΔG | ΔH | −TΔS | |
|---|---|---|---|---|---|
| Cell | Titrant | ||||
| μm | kcal mol−1 | kcal mol−1 | kcal mol−1 | ||
| ARF7PB1K1042A | ARF7PB1opca | 0.18 ± 0.03 | −9.21 ± 1.73 | −14.2 ± 0.3 | 4.98 |
| ARF7PB1K1042A | ARF7PB1opca,T1039A | 53.4 ± 1.3 | −5.77 ± 7.45 | −935 ± 24 | 929 |
| ARF7PB1K1042A | ARF7PB1opca,K1042A | ND | ND | ND | ND |
| ARF7PB1K1042A | ARF7PB1opca,G1050A | 0.68 ± 0.13 | −8.33 ± 1.62 | −9.36 ± 0.31 | 1.02 |
| ARF7PB1K1042A | ARF7PB1opca,R1051A | ND | ND | ND | ND |
| ARF7PB1K1042A | ARF7PB1opca,S1052A | 0.41 ± 0.11 | −8.63 ± 2.24 | −12.4 ± 0.4 | 3.80 |
| ARF7PB1opca | ARF7PB1K1042A,D1092A | 0.31 ± 0.02 | −8.80 ± 1.59 | −6.97 ± 0.12 | −1.83 |
| ARF7PB1opca | ARF7PB1K1042A.E1094A | 0.58 ± 0.08 | −8.42 ± 1.10 | −12.7 ± 0.3 | 4.25 |
| ARF7PB1opca | ARF7PB1K1042A,D1096A | 6.33 ± 1.67 | −7.02 ± 1.74 | −11.9 ± 1.8 | 4.90 |
| ARF7PB1opca | ARF7PB1K1042A,I1097A | 0.58 ± 0.11 | −8.42 ± 1.53 | −13.4 ± 0.4 | 5.02 |
| ARF7PB1opca | ARF7PB1K1042A,L1099A | 1.69 ± 0.23 | −7.80 ± 1.06 | −19.6 ± 0.7 | 11.8 |
| ARF7PB1opca | ARF7PB1K1042A,D1102A | ND | ND | ND | ND |
| ARF7PB1opca | ARF7PB1K1042A,D1102N | ND | ND | ND | ND |
| ARF7PB1opca | ARF7PB1K1042A,D1103A | ND | ND | ND | ND |
| ARF7PB1opca | ARF7PB1K1042A,D1103N | ND | ND | ND | ND |
| ARF7PB1opca | ARF7PB1K1042A,E1107A | 0.92 ± 0.14 | −8.15 ± 1.27 | −11.2 ± 0.4 | 3.96 |