FIGURE 10.
Guanine nucleotide exchange of H-Ras catalyzed by selected mutant SOS1cat. 50 nm wild-type or mutant SOS1cat (black or red circles)-mediated GDP for GTP nucleotide exchange upon 2 μm H-Ras (amino acids 1–166) (gray circles) in the 96-well BODIPY-FL-GDP dissociation assay as described under “Experimental Procedures.” The data are expressed as percent change of relative fluorescence units normalized to the initial time point over 30 min. Data were performed in triplicate and represent the mean.