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. 2015 Mar 30;290(20):12879–12898. doi: 10.1074/jbc.M114.634493

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FIGURE 6. — UC-773587 and UC-857993 selectively inhibit SOS1_cat-mediated versus ITSN1_DH-PH-mediated guanine nucleotide exchange activity. 10 μm UC-773587 (A) and 20 μm UC-857993 (C) inhibited 50 nm purified His₆-SOS1_cat (red circle)-mediated GDP for GTP nucleotide exchange upon 2 μm His₆-H-Ras (residues 1–166) (black circle) in the 96-well BODIPY-FL-GDP dissociation exchange assay. However, 10 μm UC-773587 (B) and 20 μm UC-857993 (D) did not inhibit 50 nm purified His₆-ITSN1_DH-PH (red circle)-mediated GDP for GTP nucleotide exchange upon 2 μm His₆-Cdc42 (residues 1–182) (black circle) in the 96-well BODIPY-FL-GDP dissociation exchange assay. Data are expressed as percent change of fluorescence units normalized to the initial time point. Data were performed in triplicate and represent the mean.