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. 2015 Mar 30;290(20):12879–12898. doi: 10.1074/jbc.M114.634493

FIGURE 7.

FIGURE 7.

UC-773587 and UC-857993 UV-visible absorbance spectra and UC-773587 fluorescence emission. The UV-visible absorbance spectrum for UC-773587 (A) and UC-857993 (B) was measured by a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). The fluorescence emission of UC-773587 in both the FL channel (C) and TR channel (D) in comparison with the fluorescence emission of exchange buffer alone and H-Ras pre-loaded with BODIPY-FL-GDP or initially loaded with BODIPY-TR-GTP as measured in the EnVision plate reader (PerkinElmer Life Sciences) (fluorescein channel: excitation, 485 nm; emission, 535 nm; Texas Red channel: excitation, 560 nm; emission, 635 nm).