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. 2015 May 15;5:10095. doi: 10.1038/srep10095

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Copyright © 2015, Macmillan Publishers Limited

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GC-FID chromatogram for the assay extracts using HP-5 capillary column (30m × 0.32 mm × 0.25 μm) (Supplemental Table S5 program 1): A) (i) Sesquisabinene synthase1 (SaSQS1) with (E,E)-FPP, (ii) Sesquisabinene synthase (SaSQS2) with (E,E)-FPP, (iii) Purified enzymatic product sesquisabinene (7). B) (i) Bisabolene synthase (SaBS) with FPP producing (S)-β-bisabolene (9), α-bisabolol (10), (ii) co-injection of (S)-β-bisabolene and assay product of SaBS with FPP. C) Santalene synthase (SaSS) with (i) FPP, (ii) GPP + IPP and farnesyl diphosphate synthase SaFDS, (iii) DMAPP + (2 × IPP) and SaFDS, α-santalene (1), β-santalene (2), epi-β-santalene (3), exo-α-bergamotene (4), exo-β-bergamotene (5), (E)-β-farnesene (6).