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. 2015 May 15;5:10131. doi: 10.1038/srep10131

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A, proteins were extracted from Aldh1a2-pET 16b positive E.coli (control), His-tag Aldh1a2-pET 16b positive E.coli (IPTG induced), purified Aldh1a2 recombinant protein, and total proteins were extracted from ovaries and testes at 30 and 90 dah of tilapia. The proteins were analyzed by SDS-PAGE followed by Coomassie blue staining. B, specific bands corresponding to the calculated molecular weights of the tilapia Aldh1a2 fusion proteins (59.8 kDa) and total proteins (56.5 kDa) extracted from ovaries and testes at 30 and 90 dah were recognized using the generated Aldh1a2 antibody by chemiluminescence. M, molecular weight markers (kDa).