Figure 8. Major cytosolic DnaJ homologues of Ssa proteins are involved in tRNA import under starvation conditions.
tRNA localization under starvation conditions in mutants of major cytoplasmic DnaJ homologues (SIS1, YDJ1 and ZUO1) or a nuclear Bag-1 homologue (SNL1) was analyzed by FISH. Pairs of YDJ1 (PJ31-3A) and ydj1∆ (JJ160) strains, SIS1 (TYSC950) and sis1-121 (TYSC951) strains, ZUO1 (BY4741) and zuo1∆ (5937) strains, and SNL1 (W303-1B) and snl1∆ (SWY1353) strains were treated as described in Figure 2 except that all the strains but the SNL1 and snl1∆ strains were cultured at 23°C instead of 30°C. In each set of panels, upper two rows are the parental wild type and the lower two rows are the mutant. Bar, 5 µm.