Fig. 6.

IDO2 ablation attenuates expression of cytokines associated with LC maturation and inflammation in CHS. (A) Cytokine levels in ear tissue. Treated and untreated ears were harvested from euthanized mice 24h after oxazolone elicitation (n = 5 each group) and cytokines were quantitated in homogenized tissue extracts by a multiplexed cytokine bead immunoassay. The data are plotted as the mean ± SEM with significance relative to baseline evaluated by the Mann–Whitney t-test. Data present the mean from three independent trials. (B) Cytokine levels in auricular lymph nodes. Treated ears were harvested as above and 1×10⁶ cells were plated and stimulated with PMA and ionomycin. Cell culture supernatants were collected 24h later and cytokine levels were quantitated as before. The data are plotted as the mean ± SEM with one replicate.