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. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Cell. 2015 May 7;161(4):845–857. doi: 10.1016/j.cell.2015.03.052

Figure 5. Eukaryotic-specific Bridges eB12, eB13 and eB14 are Differentially Affected by Inter-subunit Rotation.

Figure 5

Comparison of yeast LSU crystal structures (“ribosome A”: orange, “ribosome B”: purple) (Ben-Shem et al., 2011) with the present unrotated human atomic model (blue). The orientation aid illustrates the orientation of the 80S in each panel. (A) In the unrotated state, the C-terminal helix of eL19 forming eB12 is bend compared to the yeast structures, however, (B) virtually identical interactions are observed between eL19 and es6E. (C) To visualize the flexible linker tethering the C-terminal kinked “anchor” of eL24 forming eB13, density is shown filtered to 7.0 Å. Despite a strong displacement of the “anchor”, its overall shape remains highly similar. (D) The central bridge eB14 is hardly affected by intersubunit rearrangements. See also Table S5.