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. 2015 May 13;56(5):3004–3014. doi: 10.1167/iovs.14-15341

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Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.

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Krt12 staining of corneal whole mounts. Corneas isolated from EXT^f/f and EXT^Δ/ΔCEpi mice at P55 were analyzed by whole-mount immunostaining. (A) Krt12 (white) staining was colocalized with mTmG representing the cells from which Ext1 was ablated (green), revealing a loss of Krt12 staining in Ext1-null cells. (B) Krt12 (white) staining in the suprabasal and superficial layers of the cornea. EXT^Δ/ΔCEpi mice present a loss of Krt12 staining in the suprabasal and wing cell layers and a decrease in the superficial layer of the cornea. Scale bars: 20 μm.