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. 2015 May 13;56(5):2985–2992. doi: 10.1167/iovs.15-16466

Figure 3.

Figure 3

Lipotoxicity-mediated increase in glucotoxicity-induced increase in Nox2 is via Rac1 activation. Rac1 activation was quantified in 20 to 30 μg protein using G-LISA colorimetric assay in cells incubated in 5 mM or 20 mM glucose media supplemented with (a) 50 μM palmitate for 6 to 48 hours, and (b) with 50 μM palmitate or 50 μM ceramide for 96 hours. The results are represented as mean ± SD from three to four cell preparations, and each measurement was made in duplicate. *P < 0.05 vs. 5 mM glucose and #P < 0.05 vs. 20 mM glucose; 5 and 20 = cells in 5 mM or 20 mM glucose.