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. 2015 May 15;5:10439. doi: 10.1038/srep10439

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of REAC TO-RGD treatment on the expression of neuritogenesis regulating genes in PC12 cells. Cells were exposed from 1 (24 h) to 8 days (192 h) in the absence or presence (darker bars) of REAC TO-RGN. The amounts of β3 tubulin, neurogenin-1 NGF and tyrosine hydroxylase (TH) mRNA from REAC TO-RGN treated or untreated cells were normalized to GAPDH, and the mRNA expression of REAC TO-RGN treated cells was plotted at each time point as fold of change relative to the expression in PC12 untreated cells cultured for 24 hours after plating (named ND) defined as 1 (mean ± S.E.; n = 6). All the REAC TO-RGN treated cells at each time point were significantly different from each control untreated cells (mean ± S.E.; n = 6; P < 0.05).