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. 2015 May 15;5:10340. doi: 10.1038/srep10340

Figure 5.

Figure 5

BT treatment reduced hypertension-associated or Hcy-stimulated reactive oxygen species (ROS) over-generation in rat aortae and endothelial cells. ROS production estimated by dihydroethidium (DHE) staining in (A) en face endothelium and (B) cross-sections from control, Ang II, and Ang II + BT rats. (C) Decrease of ROS by 30 min-pretreatment of BT (5 μg/ml), TF3 (0.5 μg/ml), PBA (10 μM) or TUDCA (20 μM), followed by 45 min- treatment of Hcy in RAECs. Representative images of DHE staining in (D) en face endothelium and (E) cross-sections. Red, DHE fluorescence (excitation: 515 nm) in the nucleus; green, autofluorescence of elastin underneath the endothelium (excitation, 488 nm). Results are mean ± SEM of 4-5 separate experiments. *p < 0.05 vs Control, #p < 0.05 vs Ang II or Hcy.