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Immunolocalization of expansin and XTH epitopes in the AZs of –1 DPA to 3 DPA pollinated flower pedicels. Pollination allowed the pedicel and AZ to enlarge and strengthen in adhesion. Longitudinal sections of –1 DPA flower pedicels, including the AZ, were stained with the anti-expansin and anti-xyloglucan antibodies. Micrographs show the negative control without the first antibody step. Arrowheads indicate the AZs. Bar = 0.2 mm.
