Figure 4. Verification by PCR of CP-CSeq assignment and of clonal purity of a subset of Tn insertion mutants, following single-clone picking from library glycerol stocks.

The numbers correspond to the unique mutant ID in Supplementary Data 1 (TnInsertion-x) and a unique primer set for each mutant (Supplementary Data 2). These primers hybridize up- and downstream of the investigated transposon insertion event. PCR reactions were done both on mutant (first lane) and wild-type (WT; second lane) genomic DNA for all primer sets.