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. 2015 May 15;6:488. doi: 10.3389/fmicb.2015.00488

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Copyright © 2015 Galani, Sahuc, Njayou, Deloison, Mkounga, Feudjou, Brodin, Rouillé, Nkengfack, Moundipa and Séron.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dose-response study. Huh-7 cells were inoculated with HCVcc in the presence of increasing concentration of plant extracts (A) F3, (B) F4, and (C) F5, for 2 h. Inoculum was removed and cells were further incubated with culture medium. Infectivity was measured by the use of immunofluorescence labeling of HCV E1 envelope protein, and by calculating the number of infected cells (14.7 ± 1.6%, 17.9 ± 1.4%, 15,6 ± 1.1% of infected cells in the DMSO control condition for F3, F4, and F5 extracts dose-response assay, respectively). Data are expressed as a percentage of values obtained in the presence of DMSO. Data are means of values obtained in 3 independent experiments performed in triplicate. Error bars represent SEM.