FIG 10.

Phosphorylation- and non-phosphorylation-mimicking substitutions at H273 of PRD-1 of Mga alter M59 GAS virulence. (A) Twenty mice were infected with each indicated strain intramuscularly, and near-mortality was graphed as a Kaplan-Meier survival curve. Statistical significance between strains, as assessed by a log rank test, is indicated. (B) Histological examination of muscular lesions from mice infected with the indicated strains. The confined, less destructive lesions are indicated by arrowheads, and the areas of damaged muscle tissue with more destructive lesions are boxed. (C) Twenty mice were infected intramuscularly, and mean CFU recovered from the infected muscle tissue are shown, with P values being determined by a t test.