FIG 6.

Phosphorylation- and non-phosphorylation-mimicking substitutions at H207 and H273 of PRD-1 of Mga alter transcript levels of Mga-regulated genes. Isogenic mutant strains with single (A) or double (B) phosphorylation- and non-phosphorylation-mimicking substitutions at H207 and H273 of PRD-1 were grown to the exponential phase of growth. Transcript levels of mga, emm, scpA, and sclA in the indicated strains were measured by qRT-PCR. Four biological replicates were grown and analyzed in triplicate. Data graphed are means ± standard deviations. Values with single asterisks (P < 0.05) and double asterisks (P < 0.0005) indicate statistically significantly different transcript levels compared to the wild type.