FIG 5.

Effects of S. enterica serovar Typhimurium infection on ferroportin-1 (Fpn1), Nos2, and hepcidin (Hamp1) mRNA expression in the livers and spleens of mice. Total RNA was isolated from the livers and spleens of control (white bars) and infected (gray bars) Sv129S6 mice at 1 and 3 weeks after oral infection, and the levels of Fpn1, Nos2, and Hamp1 mRNA were assayed using real-time reverse transcription-PCR (TaqMan). The Fpn1, Nos2, and Hamp1 expression results for each sample were normalized to those of the internal control, Hprt. The fold change in target gene expression for each mouse was then calculated relative to the mean of the normalized expression level for the respective control mouse group (2^−ΔΔCT method). Means and SEM are shown; n = 3 or 4 control mice for each time point; n = 5 infected mice for each time point. +, P < 0.05; *, P < 0.001; one-way ANOVA with Tukey's post hoc test.