FIG 6.

Increased Fpn1 (brown) expression concurrent with decreased duodenal enterocyte iron (blue) staining in Sv129S6 mice after oral infection with S. enterica serovar Typhimurium. (A) Control mouse duodenum demonstrating the presence of enterocyte iron (blue). (B) Increased and basolateral (arrows) Fpn1 expression in blunted duodenal villus and concurrent loss of duodenal enterocyte iron staining 3 weeks postinfection. (C) Isotype control (without Prussian blue counterstain) showing Fpn1 (brown) and Prussian blue (ferric iron; blue) costaining; original magnification, ×50.