FIG 1.

C. perfringens biofilms are differentially produced depending on the bacterial culture medium. (A) Type C strain CN3685, or JGS1495, was inoculated into either FTG or TGY, and biofilms were incubated for 24 h. A set of wells containing biofilms were washed and photographed (bottom panel), and then the crystal violet absorbance was obtained. Asterisks indicate statistical significance (P < 0.05), as evaluated by a Mann-Whitney U test. (B) Another set of wells was stained with DAPI, and fluorescence micrographs were obtained. Experiments were repeated at least three times. (C) C. perfringens strains were inoculated in TGY medium and incubated for 24 h. Biofilms were stained with crystal violet, and the A₆₃₀ was determined. Arbitrary units obtained from CN3685 were set to 100% biofilms, and those made by the other strains were calculated. Error bars represent the standard errors of the mean calculated using data from at least three independent experiments.