TABLE 1.
Strains and plasmids utilized in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| CN3685 | Type C strain isolated from peritoneal fluid of a sheep with struck | 33, 34 |
| JGS1495 | Type C strain isolated from a piglet with necrotic enteritis | 60 |
| Strain 13 | Type A, gangrene strain | 75 |
| JGS1075 | Type C strain isolated from porcine enteritis | 76 |
| JGS1076 | Type C strain isolated from porcine enteritis | 76 |
| JGS1659 | Type C strain isolated from porcine enteritis | 76 |
| CPJV20 | CN3685ΔluxS | This study |
| CPJV21 | CN3685ΔagrB | This study |
| CPJV22 | CN3685Δplc | This study |
| CPJV23 | CN3685ΔpfoA | This study |
| CPJV25 | CN3685Δcpb | This study |
| CPJV26 | S13ΔagrB | This study |
| CPJV27 | S13Δplc | This study |
| CPJV28 | S13ΔpfoA | This study |
| CPJV30 | CPJV27 carrying pCP13plc | This study |
| CPJV31 | CPJV28 carrying pCP13pfoA | This study |
| CPJV32 | CPJV21 carrying pCPJVagrB | This study |
| Plasmids | ||
| pJIR750 | E. coli-C. perfringens shuttle vector carrying a chloramphenicol-resistant gene, catP | 54 |
| pJIR751 | E. coli-C. perfringens shuttle vector carrying an erythromycin-resistant gene, ermB | 54 |
| pJIR750ai | pJIR750 with plc targeted intron in antisense orientation | 52 |
| pCPJVlux | pJIR750ai with luxS targeted intron in an antisense orientation | This study |
| pCPJVagrB | pJIR750ai with agrB targeted intron in a sense orientation | This study |
| pCPJVpfoA | pJIR750ai with pfoA targeted intron in an antisense orientation | This study |
| pCPJVcpb | pJIR750ai with cpb targeted intron in an antisense orientation | This study |
| pCP13plc | pJIR751 carrying the plc gene from strain 13 | This study |
| pCP13pfoA | pJIR750 carrying the pfoA gene from strain 13 | This study |