TABLE 2.
Plasmids
| Plasmid | Description | Antibiotic resistance(s) | Reference or source |
|---|---|---|---|
| pLTV1 | Carries Tn917 | Emr Tcr | 19 |
| pMAD | Temperature-sensitive (30°C) E. coli-S. aureus shuttle vector; pE194ts::pBR322 | Emr | 20 |
| pBAD/HisA | Expression vector containing the araBAD promoter | Apr | 21 |
| pRMC2 | S. aureus expression vector | Apr Cmr | 22 |
| pΔmnhF | Vector for ΔmnhF mutation | Emr | This study |
| pMnhF1 | pBAD/HisA containing the mnhF internal fragment | Apr | This study |
| pMnhF2 | pRMC2 containing the mnhF internal fragment | Apr Cmr | This study |