TABLE 5.
MICs of bile salts for wild-type and recombinant E. coli strains expressing MnhF at different levels of arabinose induction
| Bile salt | MIC (mM) for strain |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wild type |
Vector control |
Recombinant |
||||||||
| TG1(pMnhF1) with: |
TOP10(pMnhF1) with: |
|||||||||
| TG1 | TOP10 | TG1(pBAD) | TOP10(pBAD) | 0% arabinose | 0.02% arabinose | 2% arabinose | 0% arabinose | 0.02% arabinose | 2% arabinose | |
| CA | 30 | 30 | 30 | 30 | 30 | 60 | 90 | 30 | 60 | 90 |
| DCA | 4 | 4 | 4 | 4 | 4 | >4 | >4 | 4 | >4 | >4 |
| CDCA | 4 | 4 | 4 | 4 | 4 | >4 | >4 | 4 | >4 | >4 |
| GCA | 50 | 50 | 50 | 50 | 50 | 100 | 100 | 50 | 100 | 100 |
| TCA | 50 | 50 | 50 | 50 | 50 | 100 | 100 | 50 | 100 | 100 |