FIG 3.

Relative effective diffusion coefficient depth profiles through uninfected and S. aureus-infected dermal explants. Relative effective diffusion coefficient is the ratio of the diffusion coefficient of water in biological material (explants) to the diffusion coefficient of water in bulk fluid (DMEM). (a) The biofilm or explant superficial surface begins at 0 μm, and the increasing negative numbers indicate increasing depths within the explants. Error bars represent SD for 3 independent NMR measurements. (b) The relative effective diffusion coefficient was significantly higher in S. aureus-infected explants than in uninfected control explants (two-way ANOVA, Holm-Šidák method; P < 0.0001; the model included explant age [4 day and 7 day] plus infection [uninfected and infected] plus interaction; the latter was not significant [P = 0.40]).