FIG 8.

Inhibition of OmpT and Pla by aprotinin. FRET assays were performed with EHEC EDL933 cells containing wild-type levels of OmpT (A) and C. rodentium ΔcroP cells producing Pla from plasmid pYCpla (B). FRET assays were performed in triplicate in the presence of increasing concentrations of aprotinin, as indicated. The percentage of omptin activity is expressed relative to the uninhibited reaction. The results are expressed as means ± the SEM of triplicate samples and are representative of three independent experiments. Statistical significance was assessed by one-way analysis of variance followed by Dunnett's post hoc test (***, P < 0.001).