Table 1.

Primers used in this study.

Primer	Sequence (5′-3′)	Restriction site
P1MutSmoR	AAGCTTTGCCCGGTTCGGTATCGG	HindIII
P2MutSmoR	GGATCCTCGCGGCGAGGCACTTCC	BamHI
P3MutSmoR	GGATCCCCGGTTCAGCGCCCGGCC	BamHI
P4MutSmoR	GAATTCCCCAGCCGCCAGCCCAGC	EcoRI
PErm5′	GGATCCGAAACGTAAAAGAAGTTATG	BamHI
PErm3′	GGATCCTACAAATTCCCCGTAGGC	BamHI
PErm5′rev	GATACTGCACTATCAACACAC	–
PErm3′rev	CTTCCAAGGAGCTAAAGAGGT	–
P1DemSmoR	GTACGTCGGGCGTATCG	–
P2DemSmoR	GCCCTTCTATGCTGG	–
P1ProSmoR	TCTAGACGCACACGCATGGACCG	XbaI
P2ProSmoR	GGATCCGAAGGCGTCGCGCTCGG	BamHI
P1ExpSmoR	CATATGAGCGATCTGGTGCAGGCG	NdeI
P2ExpSmoR	CTCGAGTCAGTCTTCGATCTCGCCT	XhoI
PT7up	TAATACGACTCACTATAGGG	–
PT7dw	GCTAGTTATTGCTCAGCGG	–