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. 2015 May 9;15:380. doi: 10.1186/s12885-015-1369-8

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© Shi et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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KPNA2 expression in urinary tract TCC cell lines and KPNA2 knockdown in vitro. (A) Western blot analyses of KPNA2 expression in normal and TCC cell lines of the urinary tract. (B) Western blot analyses of KPNA2 expression in T24 and J82 cells transfected with a control or KPNA2-directed siRNA. (C, D) Proliferation assay results from J82 and T24 cells transfected with a control or KPNA2-directed siRNA. (E) Representative images of migrating cells evaluated by the Boyden chamber assay (Original magnification: ×100). (F) The quantification results for migrating cells are presented as the mean ± SD of three independent experiments, with five random fields counted per chamber. *P < 0.05.