Overlay of spectra for tubular assemblies of CA NL4-3 A92E (orange) and CA-SP1 NL4-3 A92E (black). (A–D) Direct-DARR, (E–J) INADEQUATE, and (K) CP-DARR, acquired at 19.9 T and 4 °C. Labeled peaks indicate new resonances that appear in the CA-SP1 spectrum, but not the CA spectrum. The CP-DARR spectrum in (K) demonstrates the absence of signal from N240 of the SP1 peptide due to dynamics, while this resonance is present in the direct-DARR (C) and INADAQUATE (I) spectra. Reprinted with permission from Han et al., J. Am. Chem. Soc., 2013, 135, 17793–17803. Copyright 2013 American Chemical Society.