Table I.
Polymerase chain reaction primers for single-nucleotide polymorphisms in DPYSL2.
| SNP | Primer sequences | Annealing temperature (°C) | Product size (bp) | Restriction enzyme |
|---|---|---|---|---|
| rs9886448 (−1625T>C) | F: 5′-GAAGCCAGACTTTGAGGTGGA-3′ R: 5′-GCACTGCTTTCCAGGTTAGG-3′ |
58 | 120 | N/A |
| rs4872449 (−1195A>G) | F: 5′-CCGCACTTACTCCCCAAACA-3′ R: 5′-ACGTGTCGCGATTTGGTTTA-3′ |
52 | 317 | SmaI |
| rs431246 (−975C>G) | F: 5′-GCCCATTCCCCGCCCCCAGGAG-3′ R: 5′-CCCGCCGTTGCTGGCGCTGAAC-3′ |
70 | 169 | Alw62I |
| rs2289593 (352G>A) | F: 5′-ACCTACCGTGATCCTTCACAAG-3′ R: 5′-AGCTGGGTTACATGGATTCTTA-3′ |
53 | 327 | Fnu4HI |
| rs327222 (426C>T) | F: 5′-ACTACTCTCTGCATGTGGACATCCG-3′ R: 5′-GAGGGCATTGACTCAGTGCAACCTA-3′ |
62 | 104 | Bsh1236I |
| rs708621 (1506T>C) | F: 5′-GCTGAGCTGAGAGGCGTTCCTC-3′ R: 5′-CCTGCTGCTTGGCAGGAGACGT-3′ |
56 | 118 | BspLI |
| rs17666 (*2236T>C) | F: 5′-GTCTTCCTGTTTTTCCTGTACC-3′ R: 5′-TATTTTGCCATCAAGACAGTGG-3′ |
56 | 367 | BspTI |
N/A, not applicable; SNP, single-nucleotide polymorphisms; DPYSL2, dihydropyrimidinase-like 2; F, forward; R, reverse.