Figure 1.

Effect of pre-treatment with hydrogen sulfide (H₂S) on lipopolysaccharide (LPS)-induced cytokine production. Differentiated THP-1 macrophages were pre-treated with various concentrations of sodium hydrosulfide (NaHS), for 30 min, followed by a washout, and subsequently challenged with LPS (1 μg/ml). The levels of (A) interleukin-6 (IL-6) and (B) tumor necrosis factor-α (TNF-α) in the supernatant were analyzed at 1, 4, 8 and 24 h post-LPS challenge. NaHS exerted an inhibitory effect on cytokine production. ^*p<0.05 vs. 0, 0.01 and 0.1 mM; ^**p<0.05 vs. all groups; ^#p<0.05 vs. all groups.