Figure 3.

Effect of hydrogen sulfide (H₂S) on histone deacetylase (HDAC) and histone acetyltransferase (HAT) activity. (A) HDAC activity in THP-1 extracts treated with various concentrations of sodium hydrosulfide (NaHS) for 30 min. Trichostatin (TSA; C⁻) was used as a positive control. (B) HDAC activity was measured in the THP-1 cells pre-treated with NaHS for 30 min, followed by a challenge with lipopolysaccharide (LPS) (1 μg/ml) for 4 h. Note the lack of effect of NaHS alone (A), in contrast to the effect of NaHS pre-treatment (B), when applied prior to LPS challenge. (C) HAT activity was measured in THP-1 cells pretreated with NaHS for 30 min, followed by a challenge with LPS (1 μg/ml) for 4 h. ^*p<0.05 vs. C⁺, 0.01 and 0.1 mM; ^#p<0.05 vs. all groups; ^**p<0.05 vs. 0 mM.