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Modification of histone H3 by hydrogen sulfide (H2S). The effect of sodium hydrosulfide (NaHS) on histone H3 (A) acetylation, (B) methylation at lysine (Lys)9 and (C) methylation at Lys27 was analyzed in differentiated THP-1 cells followed by treatment with NaHS for 30 min. Cells were harvested at 4 h after the initiation of NaHS treatment for western blot analysis. Note the positive effect of NaHS on histone H3 modification. *p<0.05 vs. 0 μM.
