Figure 5.

Hydrogen sulfide (H₂S) affects lipopolysaccharide (LPS)-induced histone H3 modification at the promoter of interleukin-6 (IL-6). The (A) acetylation of histone H3 (AcH3), (B) histone H3 methylated at lysine (Lys)9 and (C) histone H3 methylated at Lys27 was analyzed by chromatin immuno precipitation (ChIP). Note that LPS alone increased the acetylation at the IL-6 gene promoter, which was attenuated by pre-treatment with sodium hydrosulfide (NaHS). In addition, pre-treatment with NaHS increased the methylation of histone H3 at the promoter of IL-6 at Lys9, but not at Lys27. ^*p<0.05 vs. LPS; ^**p<0.05 vs. control (CTL) and LPS; ^#p<0.05 vs. all groups.