Figure 6.

Hydrogen sulfide (H₂S) affects lipopolysaccharide (LPS)-induced histone H3 modification at the promoter of tumor necrosis factor-α (TNF-α). The (A) acetylation of histone H3 (AcH3), (B) histone H3 methylated at lysine (Lys)9 and (C) histone H3 methylated at Lys27 was analyzed by chromatin immuno precipitation (ChIP). Pre-treatment with sodium hydrosulfide (NaHS) on its own or together with LPS challenge reduced the acetylation bound to the TNF-α gene promoter. NaHS increased the chromatin methylation at Lys9 but not at Lys27. LPS, on the one hand, increased the association of TNF-α with the methylation of histone H3 at Lys27 but not at Lys9. NaHS pre-treatment further increased the LPS-induced of methylation histone H3 at Lys27. ^*p<0.05 vs. LPS; ^**p<0.05 vs. all groups; ^#p<0.05 vs. control (CTL) and NaHS.