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. 2015 Apr 9;35(6):1545–1553. doi: 10.3892/ijmm.2015.2178

Figure 1.

Figure 1

(A) Agarose gel image illustrating the genotyping of the transgenic mouse model. The size of the PCR product was 168 bp. The arrow indicates the 200 bp size of the molecular marker. Lanes 1,3,5 represent the genomic DNA products of 3 positive transgenic mice after genotyping and PCR amplification. Lane 2 represents the corresponding region in the wild-type littermate that had no amplified PCR fragment. Lane 4 represents the negative result of water. This animal was used as a negative control in this experiment. (B) Direct DNA sequencing to confirm the presence of mutation. The arrow indicates the corresponding methionine to valine amino acid substitution of GATA4 M310V point mutation.