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. 2015 May 8;14:68. doi: 10.1186/s12934-015-0245-1

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© Noble et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Production of SO₂ (A) and acetaldehyde (B) by the parental strains(JN10 and JN17, respectively) and the strains in which the MET2 gene of one parental strain was replaced with the MET2 allele of the other by allelic replacement(JN10 MET2 ^JN17 and JN17 MET2 ^JN10, respectively). Values are the mean of five biological replicates for SO₂ production by the parental strains, three biological replicates for SO₂ production by the hybrid, and two biological replicates for SO₂ and acetaldehyde production by the strains in which the MET2 gene of one parental strain was replaced with the MET2 allele of the other.