Table 3.

Effects of a combination of cisplatin and hyperthermia for 60 min (HT60) on cell membrane and blood gas variables in erythrocytes

Treatment	Hb (mg/ml)	P50 (mmHg)	pH	K+ (mmol/L)	Na+ (mmol/L)	ATPase (U/107 cells)	2,3-DPG (g/L)	Free Hb (mg/ml)
Control	61.68±2.69	18.58±8.76	7.44±0.17	1.43±0.48	148.71±2.74	0.078±0.022	9.67±1.74	0.089±0.005
Cisplatin (μg/ml)
50	59.58±1.61	19.36±8.45	7.43±0.14	1.96±0.51	152.58±5.63	0.069±0.020	8.92±1.12	0.091±0.005
100	58.95±5.20	21.86±7.09	7.37±0.14	2.11±0.54	153.55±3.87	0.063±0.017	8.47±1.16	0.094±0.004
200	58.47±4.76	16.91±6.20	7.26±0.09*	2.42±0.67**	154.91±6.84*	0.055±0.016*	7.63±1.22*	0.098±0.006*
HT60+cisplatin (μg/ml)
0	59.90±3.60	19.16±8.53	7.43±0.08	1.96±0.68	150.92±3.94	0.071±0.014	8.46±1.18	0.090±0.007
25	59.47±3.76	19.48±6.25	7.41±0.13	1.98±0.46	150.00±3.74	0.068±0.012	8.31±1.14	0.094±0.006
50	59.17±4.94	19.98±6.96	7.41±0.14	2.01±0.53	153.08±5.11	0.065±0.018	8.08±1.19	0.097±0.006
100	58.28±5.04	17.20±8.18	7.27±0.06*	2.33±0.58*	154.88±3.27*	0.056±0.011*	7.60±1.14*	0.104±0.008**
200	56.47±6.25	14.72±8.14	7.23±0.08**	2.64±0.61**	157.45±4.28**	0.049±0.012**	7.51±1.72*	0.106±0.008**

Data are expressed as mean±SD, with n=10 in each experiment

^* P<0.05 vs. control (treated at 37 °C for 60 min)

^** P<0.01 vs. control (treated at 37 °C for 60 min)