Table 4.

Effects of a combination of cisplatin and hyperthermia for 30 min (HT30) on cell membrane and blood gas variables in erythrocytes

Treatment	Hb (mg/ml)	P50 (mmHg)	pH	K+ (mmol/L)	Na+ (mmol/L)	ATPase (U/107 cells)	2,3-DPG (g/L)	Free Hb (mg/ml)
Control	62.20±15.87	20.28±7.81	7.45±0.11	1.46±0.56	148.28±0.46	0.080±0.012	9.67±2.09	0.089±0.008
Cisplatin (μg/ml)
100	61.93±15.66	25.78±2.88	7.42±0.09	1.91±0.51	149.33±1.23	0.069±0.015	8.94±2.07	0.092±0.008
200	60.53±14.18	27.40±3.59	7.29±0.14*	2.05±0.46	150.25±1.14	0.061±0.022	8.17±2.20	0.096±0.008
HT30+cisplatin (μg/ml)
0	63.55±14.03	25.70±3.99	7.44±0.14	1.74±0.49	148.63±0.64	0.068±0.032	9.09±2.13	0.091±0.005
100	61.73±14.03	27.00±3.71	7.35±0.16	2.12±0.68	149.95±1.16	0.059±0.023	8.01±2.10	0.099±0.008*
200	59.65±15.69	27.90±3.15	7.25±0.12**	2.29±0.60*	155.45±1.13*	0.051±0.015*	7.71±1.79	0.102±0.009**

Data are expressed as mean±SD, with n=10 in each experiment

^* P<0.05 vs. control (treated at 37 °C for 30 min)

^** P<0.01 vs. control (treated at 37 °C for 30 min)