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. 2015 May 16;15:31. doi: 10.1186/s12896-015-0152-x

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© Thompson et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NSTEM images at 40,000× magnification of H1N1 A/Puerto Rico/8/34 (A & B), H1N1 influenza-VLPs from HEK 293SF cell production (C-G) or Sf9 cell production (H-J). VLPs or influenza virus are pointed by black arrows, baculovirus and cell vesicles are pointed by white arrows. A & B - Supernatant of H1N1 A/Puerto Rico/8/1934 influenza virus produced in HEK 293SF suspension cells as in Petiot et al. [28]. Cell vesicles carrying influenza glycoproteins could be identified. C, D & E - Sucrose cushion purified samples of HEK 293SF cell production; large view (C) and zoom-in (D & E) images of NSTEM grid. F & G - Iodixanol purified samples of HEK 293SF cell production. Typical shapes of VLPs and vesicles identified in high density (fraction 12: 1.03 g/ml) and low density (fraction 2:) iodixanol fractions. H & I - Sucrose cushion purified samples of Sf9 cell production. J - Iodixanol purified samples fraction 3, which present the highest number of VLP particles. Baculovirus were co-purified with VLPs in all the iodixanol fractions, even if they were more concentrated in the high density fractions.