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. 2015 May 16;15:31. doi: 10.1186/s12896-015-0152-x

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© Thompson et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot of Sucrose cushion VLP samples from HEK 293SF and Sf9 cells. A - Anti-HA western blot with two different antibodies, a monoclonal Abs against HA2 (Santa Cruz, sc-80550) and a polyclonal antibody against HA0 (anti-HA NIBSC sheep; 03–242). B - Anti-NA western blot using anti-NA NIBSC sheep (04–230). C - Anti-M1 western blot using monoclonal mouse antibody (Abcam, ab22396).