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. 2015 May 7;15:50. doi: 10.1186/s12935-015-0202-4

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© Migliaccio et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of siRNAGAPDH on the expression levels of GAPDH mRNA. 5 × 10⁵ A20 cells were treated with A20-36-9R peptide in absence (control) or presence of siRNAGAPDH. 48 hours post-treatment, total RNA was extracted and analysed by qRT-PCR to evaluate the GAPDH mRNA expression relative to β-actin. A. Lanes: C, control; 1, A20-36-9R; 2, A20-36-9R-siRNAGAPDH; 3, A20-36-9R-siRNASCR. B. Lanes: C, control; 4, RND-9R; 5, RND-9R-siRNAGAPDH; 6, RND-9R-siRNASCR. C. Lanes: C, control; 7, siRNAGAPDH; 8, siRNASCR. Values of mean ± SD (n = 3) are shown.