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. 2015 May 15;10(5):e0126872. doi: 10.1371/journal.pone.0126872

Fig 8. Interaction of bHLH106 with a variety of G-box sequences.

Fig 8

Interactions were examined by electrophoresis mobility shift assay (EMSA). (A) Nucleotide sequences of regions containing a possible G-box were employed for EMSA as probes. (B) Thirteen kinds of 20-mer G-box sequences consisting of 5′-C/G A/GNNT/G/A G/C-3′ (M1 to M13) and arbitrary degenerate probes (C1 and C2). The “+” and “-”denote the presence or absence of bHLH106 protein in EMSA. (C) Competition experiments using the 5′-CACGTG-3′ sequences against the bHLH106 protein.